General Cell Based Assays
Revvity Health Sciences Inc HTRF FLAG Detection Kit, 500 Assay Points
HTRF FLAG Detection Kit, 500 Assay Points
Cayman Chemical Dt1 M asy Reagent 1mL
DTT (1 M) Assay Reagent has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Eurofins Discoverx cAMP Hunter eXpress NPY2R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0077E2CP2L)
The cAMP Hunter NPY2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPY2R, a naturally Gi/Go coupled GPCR. Upon activation of NPY2R, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical THUNDRcAMP TRFRET asy K 960Wel
A homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates
Eurofins Discoverx PathHunter eXpress GPR78 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0370E2ACP1M)
The PathHunter GPR78 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NADNADH Cel-Based asy KIt 1ea
Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.
Eurofins Discoverx PathHunter eXpress IL17RD/TNFR2 Dimerization Assay, 10pl Kit. (93-1068E3CP5L)
The PathHunter IL17RD/TNFR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TNFα) induced dimerization of IL17RD/TNFR2 receptors. This product uses EFC technology. The cell line included overexpresses IL17RD target protein fused to PK alongside TNFR2 target protein fused to EA. Upon ligand-induced dimerization of IL17RD/TNFR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPSR1b U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0822E3CP0M)
The PathHunter NPSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide S) based activation of NPSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1b and EA-tagged β-Arrestin-2. Activation of NPSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 2-Plate. (95-0118Y2-00057)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Bioassay Systems EnzyChrom™ HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Requires only 20 µL serum sample. Detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD340nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 5 mg/dL.
Revvity Health Sciences Inc HTRF Mouse STING WT Binding Kit, 10,000 Assay Points
HTRF Mouse STING WT Binding Kit, 10,000 Assay Points
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 1 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 100 mL kit enables user to run 5000 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Cell Biolabs Inc OxiSelect™ AOPP Assay Kit, 200 assays
Oxidative stress is defined as an increase in the production of reactive oxygen species (ROS) due to an imbalance between antioxidant and oxidants. Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of chlorinated oxidants, such as chloramines and hypochlorous acid, with plasma proteins. AOPPs are structurally similar to Advanced Glycation End-Product (AGE) proteins and exert similar biological activities. The OxiSelect™ AOPP Assay Kit offers a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates. This kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, U2OS, 10pl Kit. (93-1000E3CP5L)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human HSP27/HspB1 ASY
The R-PLEX Human HSP27 (HSPB1) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures human HSP27 protein levels in serum plasma cell culture supernatants cell and tissue lysates and other biological samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls This assay allows for the quantitative measurement of HSP27 levels with high precision and reproducibility